FST 202/2042 GENERAL MICROBIOLOGY       

UNIT I

History and Scope of Microbiology. The discovery of Microorganism. Spontaneous generation versus biogenesis, the germ theory of disease, pure culture concept, structural organization of prokaryotic cells and eukaryotic cells . Cultivation of Microorganism: Types of culture media, synthetic and complex media, sterilization, aseptic transfer, isolation and incubation, Microbial nutrition (Requirements for C, H, O, N, P and S), nutritional types of Micro-organism, Isolation of pure culture (spread, streak and pour plate method).

UNIT II

Survey of Micro-Organism : A brief account of the classification of microorganisms.       A general account on Virus, Bacteria, Algae, Fungi and Protozoa.

UNIT III

Microbial Metabolism: Chemical principles of metabolism, autotrophic and heterotrophic metabolism. Transport mechanism, Nitrogen metabolism and fixation. Biogeochemical cycles – Carbon cycle, Hydrogen cycle, Nitrogen cycle, Oxygen  & Sulphur cycle

UNIT IV 

Microbial Growth : The growth curve, Continuous culture of Microorganism. Factors influencing Microbial growth (Oxygen, water, pH, temperature, pressure, salinity). Physical and chemical control of Microorganisms, Antibiotics as chemotherapeutic agents.

UNIT V

Microorganisms and Human Diseases: A general account of disease causing microorganisms in humans- Respiratory tract diseases, Genitourinary tract diseases, Sexually transmitted diseases. Normal micro flora of human body.

REFERENCES

·         Microbiology – An Introduction by Tortora, Funke and Case.

·         An Introduction to Microbiology  by Palczar.