FST 201/2041 GENERAL BIOCHEMISTRY
Unit-I
Properties of water, weak acids & weak bass, pH & buffers.
Carbohydrates:- Classification, Killani synthesis, mutarotation, structures and importance of Mono, Oligo and polysaccharides, Maltose, Lactose, Sucrose, Isomaltose, glycogen starch, Homo and Hetro, polysaccharides, Proteoglycan, Mucopolysaccharides.
Lipids. Simple and compound lipids, Phosphogycerides sphingolipids, terpens, sterols and Ecosanoides structure of Biological membrane.
Unit-II
Amino Acids;- Chemical structure general properties and titration curve.
Proteins:- Structure of peptides, peptide bond, protein structure primary, secondary, Tertiary and Quaternary structure of protein, Forces stabilize the protein structure , salting out , salting in, Denaturation and renaturation.
Unit-III
Enzymes Nameculture, classification: Michelis Menten equation, Line -Vier burk plot competition, non competition and un competition inhibition, enzymes catalysis, substrate enzyme reaction, pro-enzyme and enzyme activation- Allosteric enzyme co-operativity. Importance of enzyme. Nucleic Acid:- Structure of Nucleic acid (DNA & RNA)) Nucleotides & nucleosides, different forms of DNA, Denatruration and renaturation.
Unit-IV
Carbohydrates Metabolism :-Glycolysis fermentation, gluconeogensis Glylycogenolysis, Pentose phosphate pathway: TCA cycle, Gatyoxylate pathway Electron transport chain.
Lipid Metabolism:- Digestion and absorption, β,& ώ Oxidation of fatty acids, , fatty acid Biosynthesis, Ketone body formation, cholesterol biosynthesis, Urea Cycle
Unit-V
Molecular Biology & Biotechnology Replication of DNA (E. coli), transcription & translation, Recombinant DNA technology, plasmid, cosmid, Phage vector, Genomic and cDNA Library, Southern Northern, and western blotting.
REFERENCES
1. Biology for Chemist by Agrawal & Agrawal.
2. Biochemistry by Albert L Lehninger.
3. Biochemistry by U Satyanarayana &U Chakrapani
4. Fundamentals of Biochemistry by J L Jain, Sunjay Jain & Nitin Jain.