FST 154/6294  BIOCHEMISTRY

Unit I  (10 Lectures)

Properties of water, weak acids, weak bases, pH and buffer. Carbohydrates: Classification, killani synthesis, mutarotation, structure and importance of mono, oligo and polysaccharides. Maltose, lactose, sucrose, isomaltose, glycogen,starch, homo and hetero polysaccharides, proteoglycan, muco-polysaccharides. Lipids: simple and compound lipids, phosphoglycerides, sphingolipids, terpens, sterols, and ecosanoides structure of biological membrane.

Unit II  (7 Lectures)

Amino acids: Chemical structure, general properties, and titration curve. Proteins: Structure of peptides, peptide bond, protein structure (primary, secondary, tertiary and quaternary structure of proteins). Forces stabilizing the protein structure, salting out, salting in, denaturation, and renaturation.

Unit III  (8 Lectures)

Enzymes: Nomenclature, classification, Michelis Menten equation, line-vier burk plot competition, non competition and competition inhibition, enzyme catalysis, substrate enzyme reaction, pro-enzyme and enzyme activation- allosteric enzyme co-operativity. Importance of enzyme. Nucleic acid: structure of nucleic acid (RNA and DNA), nucleotides, nucleosides, different forms of DNA, denaturation and renaturation.

Unit IV (8 Lectures)

Metabolism: Carbohydrate metabolism; glycolysis, fermentation, gluconeogenesis, glycogeneolysis, pentose phosphate pathways, TCA cycle, gatyoxylate pathway, electron transport chain. Lipid metabolism: digestion, absorption, alpha, beta and omega oxidation of fatty acids. fatty acid biosynthesis, ketone body formation, cholesterol biosynthesis, urea cycle. Nitrogen metabolism and secondry metabolism. 

Unit V(7 Lectures)

Molecular biology and biotechnology: replication of DNA, trancription,translation, recombinant DNA technology, plasmid, cosmid, phage vector, genomic and cDNA library, southern, northern and western blotting.

REFERENCES

1.               Biochemistry by Albert L Lehninger.

2.               Biochemistry by Jeremy M Berg.

3.               Biochemistry by U Satyanarayana & U Chakrapani.

4.               Fundamentals of Biochemistry by J L Jain, Sunjay Jain & Nitin Jain.