FST 153/6293  MICROBIOLOGY

Unit I (10 Lectures)

History and Scope of Microbiology, The discovery of Microorganism, Spontaneous generation versus biogenesis, the germ theory of disease, pure culture concept, Structural organization of prokaryotic cells and eukaryotic cells. Cultivation of Micro-organism: Types of culture media, synthetic and complex media, sterilization, aseptic transfer, isolation and incubation. Microbial nutrition (Requirements for C, H, O, N, P and S), Nutritional types of Micro-organism, Isolation of pure culture (spread, streak and pour plate method).  

Unit II  (10 Lectures)

Microbial Metabolism: Chemical principles of metabolism, autotrophic and heterotrophic metabolism. Transport mechanism. Ecological aspects of microbial metabolism. Biogeochemical cycles - carbon, hydrogen nitrogen & sulphur cycles.            Microbial Growth : The growth curve, continuous culture of Microorganism. Factors influencing Microbial growth (Oxygen, water, pH, temperature, pressure, salinity). Physical and chemical control of Microorganisms, antibiotics as chemotherapeutic agents.  

Unit III  (5 Lectures)

Survey of Micro-Organism : A brief account of the classification of microorganisms.         A general account on virus, bacteria, algae, fungi and protozoa.

Unit IV  (6 Lectures)

Microorganisms and Human Diseases: A general account of disease causing microorganisms in humans- Respiratory tract diseases, Genitourinary tract diseases, Sexually transmitted diseases. Normal micro flora of human body.

Unit V  (7 Lectures)

Applied Microbiology: Major Products of Industrial Microbiology. Amino Acid, Organic Acid, Bio conversion process. Fermentation: Lactic acid fermentation, acetic acid fermentation, alcoholic fermentation, Malo-lactic fermentation.

REFERENCES

·         Microbiology – An Introduction by Tortora, Funke and Case.

·         An Introduction to Microbiology  by Palczar.